Physical mapping of the 18S-25S and 5S ribosomal RNA genes in diploid bananas
1998
Dolezelova, M. (Akademie Ved, Olomouc (Czech Republic). Ustav Experimentalni Botaniky) | Valarik, M. | Swennen, R. | Horry, J.P. | Dolezel, J.
Fluorescent in situ hybridisation (FISH) was used to determine the number and distribution of the 18S-25S and 5S rDNA sites on mitotic chromosomes of 6 wild and 2 edible diploid (2n=22) accessions belonging to the two banana species, Musa acuminata and M. balbisiana. FISH with the 18S-25S probe resulted in signals on one pair of chromosomes, the position of signals corresponded to the secondary constriction at the end of a short arm. The intensity of labelling was different between the homologues and the larger site corresponded to a larger secondary constriction. This labelling pattern was observed consistently in all genotypes.
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