Isolation, fractionation and HPLC analysis of neutral phenolic compounds in rapeseeds
1998
Matthaus, B.
HPLC has been applied for the analysis of neutral phenolic compounds in rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic by solid phase extraction with a C18-cartridge and HPLC separation of the extracts so obtained are described. Additionally, a rapeseed extract was fractionated by a simple chromatographic method using Sephadex LH-20. Six main compounds from the extract were isolated. A further purification was performed by preparative HPLC and a C18-cartridge are used to isolate the neutral phenolic compounds. An attempt to identify the compounds was made from the UV-spectra corresponding to each isolated peak of the chromatogram.
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