Enrichment of phosphatidyl choline - alcohol fractionation of carbon dioxide de-oiled lecithin from soybean (Glycine max)
1997
Began, G. | Manohar, B. | Udaya Sankar, K.
Crude soylecithin was extracted with carbon dioxide at pressures of 120, 200, 240, 280 and 320 bar (12, 20, 24, 28, and 32 MPa, respectively) and at a temperature of 40 deg. C to obtain de-oiled lecithin. The amount of phospholipids (acetone insoluble) increased with increasing pressure. The carbon-dioxide-extracted lecithin (CDEL) was fractionated with alcohol into alcohol-soluble and alcohol-insoluble fractions. Depending on the pressure, the alcohol-soluble fraction of CDEL was found to contain up to 70% phosphatidyl choline as against 21.1% present in crude lecithin. The alcohol-insoluble fraction of CDEL was found to contain up to 37.1% phosphatidyl inositol as against 12.3% in crude lecithin. The chemical parameters of CDEL and the alcohol-soluble and alcohol-insoluble fractions were evaluated.
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