High pressure freezing and freeze substitution reveal new aspects of fine structure and maintain protein antigenicity in barley aleurone cells

aleurone cells aleurone layer antibodies anticorps anticuerpos antigene antigens aparato golgi appareil de golgi barley capa de aleurona cebada cell membranes cell structure citoplasma cytoplasm cytoplasme dehydration drying electron microscopy estructura celular freezing golgi apparatus hordeum hordeum vulgare membranas celulares membrane cellulaire microscopia microscopie microscopy microtubule microtubules mitochondria mitochondrie mitocondria noyau cellulaire nucleo nucleus orge proteine proteins protoplaste protoplastos protoplasts secado structure cellulaire vacuole vacuoles