Effect of ZnSO4 and CuSO4 on regeneration and lepidine content in Lepidium sativum
2000
Saba, D. | Pande, M. | Srivastava, P.S. (Hamrad Univ., New Delhi (India))
Significant amounts of lepidine was detected in mature and juvenile explants from both in vivo and in vitro grown plants. The yield, however, was variable depending upon the source and type of explant used. Mature in vivo plants at vegetative stage exhibited highest yield. Among all the explants, maximum lepidine was detected after 8 weeks in shoot apex callus on MS medium supplemented with 2 mg dmE(-3) naphthaleneacetic acid and 5 mg dmE(-3) benzylaminopurine. Addition of 900 micro M Zn2+ or 100 micro M Cu2+ further enhanced the yield of lepidine.
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