Root induction in microshoots of Simarouba glauca L-in vitro: Peroxidase as a marker for rooting
1999
Rout, G.R. | Samantaray, S. | Das, P.
Induction of rooting in microshoots of Simarouba glauca. L. was achieved within 12 to 15 days of culture on MURASHIGE and SKOOG's (1962) medium supplemented with 1.0 mg/l IBA and 3% (w/v) sucrose. There was no spontaneous rooting observed without the application of auxins. Peroxidase activity was the minimum at the induction phase and maximum at the initiation and expression phase grown on medium containing 1.0 mg/l IBA. Rooting was associated with selective expression or repression of isoforms of peroxidase during induction, initiation and expression phase. This study indicates a key role of peroxidase in rooting of microshoots of Simarouba glauca in vitro.
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