Differentiation of cereal eyespot fungi using morphologic characteristics and PCR diagnostic tests
2000
Vanova, M. | Polisenska, I. (Zemedelsky Vyzkumny Ustav, Kromeriz (Czech Republic)) | Vejl, P. | Skupinova, S.
Stem parts of winter wheat infected with eyespot caused by Pseudocercosporella herpotrichoides were collected at various locations in the Czech Republic in 1995-1998. Pure colonies of the fungi were cultivated on potato-dextrose agar, visually classified into the teleomorphs and tested for sensitivity to MBC-fungicides. Selected isolates of Tapesia yallundae and T. acuformis were examined using RAPD markers. Of the total number of 1,014 isolates collected, 70 % were assessed as W-type (T. yallundae) and 30 % as R-type (T. acuformis). The frequencies of T. yallundae/T. acuformis in each of the 4 years were 73/27, 78/22, 65/35 and 66/34 percent. Among five tested primers, only 5'GATGACCG 3'provided polymorphic RAPD products at minimum non-specific amplification.
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