Biochemical monitoring of aquatic pollution: Indicators of dioxin-like toxicity and oxidative stress in the roach (Rutilus rutilus) and chub (Leuciscus cephalus) in the Skalice river [Czech Republic]
2000
Machala, M. | Ulrich, R. | Neca, J. (Vyzkumny Ustav Veterinarni Mediciny, Brno (Czech Republic)) | Vykusova, B. | Kolarova, J. | Machova, J. | Svobodova, Z.
Concentrations of major classes of organic contaminants were determined in muscle samples and biochemical markers indicating dioxin-like toxicity and oxidative stress were measured in liver samples collected from roach (Rutilus rutilus) and chub (Leuciscus cephalus) fished out at nine sites of the Skalice river (Czech Republic). No significant differences were found in the concentrations of polychlorinated biphenyls (PCBs) and organochlorine insecticides between the two species. The concentrations of polycyclic aromatic hydrocarbons were higher in roach than in chub. The data on PCB concentrations in muscle tissue correlated well with the induction of the CYP1A-dependent 7-ethoxyresorufin O-deethylase activity (EROD) as the biomarker of the dioxin-like toxicity in the hepatic microsomal fraction of roach and chub. Changes in the hepatic microsomal glutathione-S-transferase and cytosolic glutathione reductase activities were used as selective biomarkers of oxidative stress.
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