MALDI-MS characerization of maltooligo/polysaccharides from debranched starch amylopectin of corn and barley
1999
Wang, J. | Jiang, G. | Vasanthan, T. | Sporns, P.
A protocol was developed for molecular characterization of debranched starch amylopectin using Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Starch from waxy corn was used in the protocol development. Various factors influencing the protocol were investigated and discussed. Using the developed protocols, starch amylopectin from barley grains (waxy-type Candle and regular-type Phoenix) were also characterised. The study suggested that MALDI-MS is suitable to determine the molecular size profile of debranched starch (maltooligo- and polysaccharides) faster and with greater accuracy than any present analytical methodology.
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