Chromium, nickel and zinc tolerance in Leucaena leucocephalla (K8)
1999
Rout, G.R. | Samantaray, S. | Das, P.
In vitro shoot bud regeneration via callogenesis was achieved from hypocotyl explants of Leucaena leucocephalla (K8) collected from both contaminated (chromium, nickel and zinc) and uncontaminated sites. The proliferated calli raised from hypocotyl were tested in vitro for their relative tolerance to chromium, nickel and zinc. The calluses derived from contaminated sources were tolerant to metal and showed better growth on MS basal medium supplemented with 1.0 mg 1(-1) kinetin, 4.0 mg 1(-1) NAA and either 0.015 mM chromium, 0.13 mM nickel or 0.053 mM zinc than those derived from uncontaminated seeds. The specificity of metal tolerance noted in the parent materials was observed in the calluses. The calli derived L. leucocephalla growing on contaminated soil exhibited higher catalase and peroxidase activities than those from the uncontaminated soil. Biochemical studies provided evidences that plant material from the contaminated sources were physiologically distinctfrom the uncontaminated ones. This study indicated that seeds of Leucaena leucocephalla collected from contaminated sites were tolerant to chromium, nickel and zinc and may have the advantage of being used in sustainable revegetation programmes on metalliferous minewastes.
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