Prospects and possibilities of buckwheat development through biotechnology
2001
Joshi, B.K. | Bimb, H.P.(Agricultural Botany Division, Khumaltar, Lalitpur (Nepal))
Genetic manipulation of buckwheat is very difficult due to its unique reproductive biology. In vitro techniques together with conventional breeding methods could be used to speed up the breeding program and overall improvement of buckwheat. Suggestion is made using plant cell and tissue culture techniques as means to overcome breeding barriers in buckwheat, a difficult crop not only in terms of conventional breeding but in terms of unconventional breeding as well. Success in some extent are reported in buckwheat improvement using biotechnological tools such as anther and ovule culture, callusing and somatic embryogenesis, interspecific hybrids using embryo rescue, protoplast isolation and somatic hybridization, genetic engineering. Major genetic problems are self-incompatibility due to dimorphic heterostylism, which is controlled by single gene; incomplete reproductive organ mainly in the female; failure of fertilization and seed collapse in the post zygotic stage. The problems encountered in the buckwheat development may be solved using the biotechnological tools especially by developing interspecific hybrids, transgenic plants and new populations. Achievements made at the international level on biotechnological research of buckwheat give scope to widen the research area on buchwheat in Nepal.
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