Isolation and structural identification of Molluscicidal compounds from the leaves of Pittosporum tobira
1999
Diab, Y. M.
The objective of this work was to extract and isolate the active molluscicidal compound(s) from the leaves of Pittosporum tobira at this plant met the selection criteria proposed by WHO for viable plant molluscicides. The isolation was carried out by using a combination of different chromatographic methods (TLC and column chromatography). The purity of the isolated compound was established by its resolution as a single spot in four different TLC systems. The biological evaluation of the extracts and isolated compound(s) was carried out against Biomphalaria alexandrina snails. The greater part of the molluscicidal activity of the air dried leaves of Pittosporum tobira was a methanolic extract. The purified saponin from methanolic extract was characterized by chemical methods (detection tests, acidic hydrolysis and acetylation of isolated compound) and spectroscopic methods GC-MS and H1,C13 NMR
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