A study on Citrus infectious variegation virus (CVV) in Citrus from Gargano promontory, ltaly
2004
Daccache, G.
Leaf variegation and crinkle-Iike symptoms were observed in most of the local citrus species on Gargano promontory in ltaly. Therefore, 30 accessions from Gargano and 3 CVV isolates were selected for biological, serological and molecular characterization of citrus infectious variegation virus (CVV). Inoculated woody plants showed mainly leaf crinkling and blister-like symptoms. High CVV transmissibility to herbaceous hosts was observed and Nicotiana cavicola was a new virus host. TAS-ELISA, using the commercial kit, and 4 monoclonal antibodies revealed high CW infection and variability, which clustered the 23 CVV-infected sources in 5 serogroups. The CVVa/CVV4 couple of primers could detect a higher number of CVV isolates than CVVa/CVVb and serological assays; heterogeneity was observed by SSCP results. Seed coats were highly infected unlike the progeny. Slice inoculations from citrus to indicators highly transmitted the virus. DTBIA using ovaries proved to be more effective than ELISA. The Southern side of the tree was chosen for sampling and CVV incidence in Gargano was 30 percent.
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