Extraction of plant proteins for two-dimensional electrophoresis

Granier, Fabienne

Extraction of plant proteins for two-dimensional electrophoresis

1988

Granier, Fabienne

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.

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