Nanospider technology for controlling of Pseudomonas cichorii and Dickeya dadantii by electrospun nanofibers of nylon-6/chitosan blends
2014
Abdel-Megeed, Ahmed (Faculty of Agriculture - Saba Basha, Alexandria (Egypt). Department of Plant Protection) | Eifan, Saleh A. (King Saud University, Riyadh (Saudi Arabia). College of Science, Department of Botany and Microbiology) | El-Newehy, Mohamed Hassan (King Saud University, Riyadh (Saudi Arabia). College of Science, Department of Chemistry) | Al-Deyab, Salem S. (King Saud University, Riyadh (Saudi Arabia). College of Science, Department of Chemistry)
This is the first report on the use of electrospun nanofibers which could be of considerable interest to the development of new antibacterial compounds against two species of bacteria: Pseudomonas cichorii causing bacterial leaf spot (bacterial midrib rot) and Dickeya dadantii (Erwinia chrysanthemi) causing bacterial bligh. Electrospun nylon-6/chitosan (nylon-6/Ch) nanofibers were obtained using formic acid as a single solvent. Surface modification of electrospun nylon-6/chitosan nanofibers was performed by soaking the mat in an aqueous solution of glycidyltrimethylammonium chloride (GTMAC) at room temperature overnight to give nylon-6/N-[(2-hydroxy-3-trimethyl ammonium)propyl] chitosan chloride (nylon-6/HTCC). The morphological, structural and thermal properties of the nylon-6/chitosan nanofibers were studied by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, and thermogravimetric analysis (TGA). Biological screening demonstrated that Nylon-6/HTCC mat exhibited high potential antibacterial activity on protein synthesis of bacteria Pseudomonas cichorii and Dickeya dadantii. Bacteria examined using SEM were totally deformed and exhibited symptoms of severe destruction.
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