Morfološka karakterizacija polenovih zrna sorti badema pomoću skenirajuće elektronske mikroskopije | Morphological characterision of pollen grains of almond cultivars using scanning electron microscopy
2022
Milatović, Dragan | Nikolić, Dragan | Radović, Aleksandar
Morphology of pollen grains was studied in eight almond cultivars using scanning electron microscopy (SEM) technique. The following characteristics were examined in dried pollen grains: length (L), width (W), L/W ratio, length of the colpus, width of the mesocolpium, and exine characteristics (number of ridges per a 100 μm2 area, width of a ridge, and width of a furrow). Statistically significant differences between cultivars for all studied traits were found. All cultivars had isopolar, radially symmetric and tricolpate pollen grains, which had been classified as large in size. Pollen grain length ranged from 56.21 μm in cultivar ‘Prianiy’ to 60.45 μm in cultivar ‘Retsou’, and width from 30.08 μm in cultivar ‘Ferragnes’ to 32.49 μm in cultivar ‘Retsou’. Pollen shape was identified as prolate in all cultivars, with L/W ratio from 1.83 to 1.90. The length of the colpus varied in the range of 50.61‡54.34 μm, and the width of the mesocolpium of 15.77‡17.62 μm. All studied cultivars had striate exine ornamentation. Number of ridges per 100 μm2 of the exine surface was the lowest in cultivar ‘Nessebar’ (17.7), and the largest in cultivar ‘Icar’ (24.7). Ridge and furrow width varied in a range of 0.31‡0.49 μm and of 0.29‡0.43 μm, respectively. SEM examination indicated that some morphological characteristics of pollen grains, such as size, shape, and exine ornamentation, can potentially be used in the identification of almond cultivars.
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书目信息
出版者 Scientific Pomological Society of Serbia, Čačak (Serbia)
ISSN 1820-5054