Study of the soybean specific retrotransposon (SIRE1) in wheat (Triticum aestivum L.) genotypes grown in vivo and in vitro
2022
Transposon mobility and polymorphism ratios were examined in leaf samples obtained from a wheat plant (Triticum aestivum L.) in vitro callus culture, in vivo seeds and regenerants . The callus mass was cultured in Murashige and Skoog nutrient medium for 10 and 20 days. The seeds, and regenerating leaves were grown in the soil for 10 and 20 days and leaf samples were obtained. Soybean-specific SIRE1 retrotransposons were detected in seeds, callus, and regenerating leaves of wheat, and different polymorphism results were obtained in each sample. The mobility of retrotransposons was determined in Azamatli 95, Barakatli 95, and Gobustan wheat genotypes using the IRAP method. SIRE1 IRAP analyses resulted in 432 bands ranging from “300 to 2500” bp. It has been observed that 244 bands are polymorphic, and 188 bands are monomorphic. This work is the first epigenetic study conducted on wheat (in vitro callus, in vivo seed and rejenerant leaf) plant in Azerbaijan.
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