Cultivation of Pleurotus ostreatus and Lentinus edodes on Linocellulosic Substrates Tes for Human Food and Animal Feed Production

Ligninolytic microorganisms, mainlywhiterootfungi are able ta colonize different plant residues and increase the digestibility. Lignocellulosic w",tes can also be usedfor the cultivation ofediblemushrooms. Therefore, thisstudy w'" set up ta assess thesuitability ofwheatstrawandsugarcane bagassesupplementedwith variouslevelsof wheat bran, a, substratesfOr 2fungispecies. Milledwheatstrawandsugarcanebagasse were placed in jars and supplemented with 00/0, 5%, 100/0 and 15% of wheat bran. Deionized water (75~) w'" added to thejars, which were then sterilized (121°C; 30 min). The substrateswereinoculatedwithmyceliumofPleurotus ostreatusand Lentinus edodes, and then incubated(25°C; 45 days). During the colonization period, the pH, water soluble substances, organic matter (OM) loss, lignin content and in vitro digestibility(IVD)ofthesubstrateswere measured. Afterthecolonizationperiod; thejars were placed in an incubator (l8°C; RH 80-900/0) fOr fructification. Thefruiting badies were collecteduntil150days. The IVD ofspentsubstrate aftermushroom cultivation w'" measured Generally, thefungi colonizedbetterwheatstrawthansugarcane bagasse. P. ostreatus degradedOM ofsubstrate and ligninfasterthanL edades. Thehighestlassof organicmatterofwheatstrawcfter incubation wUhP.ostreatusand L edadesfor 60 day: were 28.9% and 23.7% respectively. The substrate pH w'" lower on substrate incubated by L edodesthanby P.ostreatus (.1.8 vs 4.3). II w'" corresponding with the watersolublesubstances ofsubstrates. Thedigestibility ofbothsubstrates w'" 3.8% 24.2% higher than the control. The supplementation of wheat bran on the substrates increasedthefruitingbodiesyield TheyieldofP.ostreatusgrowingonwheatstrawwith supplementation of15% wheatbranw'" highest (8.3% inDM). Theinvitrodigestibility of spent substrate after cultivation of 1. edodes w'" 68% The upgrading of lignocellulosicwastesintofoodandanimalfeedis possible.However, theappropriate technologyfor thisbioconversion isneeded.