Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
2015
FAJARDO, T. V. M. | NASCIMENTO, M. B. | EIRAS, M. | NICKEL, O. | PIO-RIBEIRO, G. | THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.
ABSTRACT There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group. Key words: diagnosis, sequencing, Ilarvirus, PNRSV, ApMV, ArMV RESUMO Inexiste caracterização molecular de isolados brasileiros de Prunus necrotic ringspot virus (PNRSV), exceto aqueles de pessegueiros. Neste trabalho, o agente causal do mosaico da roseira foi determinado e os genes das proteínas de movimento (MP) e capsidial (CP) de um isolado de PNRSV de roseira foram molecularmente caracterizados pela primeira vez no Brasil. As sequências completas de nucleotídeos e de aminoácidos deduzidos da MP e da CP foram alinhadas e comparadas com outros isolados. Análises das sequências de nucleotídeos da MP e da CP do isolado brasileiro de PNRSV de roseira e outros isolados da mesma hospedeira revelaram altas identidades de 96,7% e 98,6%, respectivamente, sendo o isolado Rose-Br classificado no grupo PV32. Palavras-chave: diagnose, sequenciamento, Ilarvirus, PNRSV, ApMV, ArMV.
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