Susceptibility of Human B Lymphocyte cell lines to dialysed latex B- and C-sera fractions
2012
Chow T. W. | Yang K. L. | Ong M. T. | Lam K. L. | Chai Y. L. | Sunderasan E
Hevea brasiliensis latex sera are being investigated for anticancer property. In the present cell-based assay, whole latex B- and C-sera were shown to be largely ineffective against human B lymphocytes, of both cancer origin (BDCM) and non-cancer origin (Bristol 8); albeit slight dip in cell viability were discerned at high concentrations (>100 μg/mL) of whole C-serum, and (>200 μg/mL) of whole B-serum. Interestingly, the antiproliferative activity improved in the retentate of dialysed C-serum, the increment however was more prominent in the dialysed C-serum precipitate (DCP) compared to the dialysed C-serum supernatant (DCS). DCP also showed a marginally higher specificity against BDCM (LC50 = 55.95 μg/mL) compared to Bristol 8 (LC50 = 75.62 μg/mL). However in the treatments with the retentate of dialysed B-serum, both BDCM and Bristol 8 cells remained unaffected.
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