Resveratrol-enriched transgenic rice callus extract (IS526) causes inflammation via the MAPK pathways in rabbit articular chondrocytes
2020
Eo, S.H. | Kim, S. J.
Osteoarthritis is a common degenerative joint disease. Resveratrol-enriched rice (Iksan526) was used in the present work to investigate the effects of IS526 callus extract (IS526) on inflammatory mediators in rabbit articular chondrocytes. Prostaglandin E₂ (PGE₂) was detected by an assay kit. Protein levels of cyclooxygenase-2 (COX-2), extracellular signal-regulated kinases (ERK)1/2, and p38 kinase were measured by western blotting. IS526 induced the expression of COX-2 and PGE₂ . In addition, after treatment with IS526, both p38 and ERK1/2 were phosphorylated. Inhibiting ERK1/2 and p38 kinase with PD98059 and SB203580 suppressed IS526-stimulated PGE₂ and expression of COX-2, respectively. These findings suggest that IS526 induces inflammation via the p38 kinase and ERK1/2 pathways in rabbit articular chondrocytes.
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