Evaluation of minor steviol glycosides effect on insulin resistance, serum triglycerides, and antioxidant capacity of diabetized Wistar rats
2021
Betancur-Ancona, D. A. | Coop-Gamas, F. Y. | Aranda-González, I. I. | Diaz-Canul, C. | Ávila-Escalante, M. L.
The objective of the present work was to evaluate the effect of minor glycosides on the QUICKI index as a marker of insulin resistance, triglycerides (TG), and antioxidant capacity in Wistar rats induced with diabetes mellitus type 2 (DM2). DM2 was induced in male Wistar rats (n = 35) through streptozotocin-nicotinamide. Hyperglycaemia was confirmed two weeks later, the subjects were divided into seven experimental groups, and each group was treated as follows: (1-5) dulcoside A, steviolbioside, rebaudioside B, C, and D (20 mg/kg, respectively); (6) metformin (180 mg/kg); and (7) standard diet, orally for four weeks. Blood sample was obtained from the tail before and after the treatment. The serum was separated after clotting by centrifugation. The included parameters namely serum triglycerides (TG) and superoxide dismutase (SOD) activity were measured before and after the treatments, then the changes were determined; and at the end of the treatment, the QUICKI index was determined. The analysis of one-way variance (ANOVA) was performed considering p < 0.05. No statistically significant differences were found in any of the three variables (p > 0.05); however, the rebaudioside group B had the highest QUICKI index, while the reduction of triglycerides was greater in rebaudioside D. SOD activity increased in all groups, but was higher in rebaudioside D and steviolbioside. Minor glycosides at the dose and time evaluated had no significant effects on QUICKI index, antioxidant capacity, and triglycerides concentration.
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