Development and characterisation of oil palm (Elaeis guineensis Jacq.) microsatellite markers
2006
Barcelos, E. | Risterucci, Am | Noyer, J. L. | Amblard, P. | Billottte, N. | Baurens, F. C.
A large Elaeis guineensis microsatellite development program has been undertaken to generate a wide range of (GA)n, (GT)n and (CCG)n Simple Sequence Repeats (SSR) markers useful for oil palm genetic mapping, genetic diversity study and variety identification. Comparative results in building several microsatellite-enriched libraries demonstrate that an optimised hybridisationbased capture methodology, using biotin-labelled microsatellite oligoprobes and streptavidincoated magnetic beads, is the quickest and best adapted procedure to ensure a high enrichment rate with minimal redundancy. A set of 21 SSR loci were evaluated with similar estimates of allele size range and expected heterozygosity in E. guineensis as in the closely related E. oleifera species where an optimal transportability of the SSR markers was observed. SSR mendelian segregation allows oil palm breeders to use SSR markers for genetic mapping and for the search of agronomic genes based on both intra- or inter-specific progenies. Multivariate data analysis show the ability of our developed SSR markers to reveal the genetic diversity structure of E. guineensis and E. oleifera natural populations in accordance with already measured relationships and with geographical origins. Very high levels of allelic variability indicate that SSRs will be apowerful toolfor variety identification in Elaeis species.
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