Evaluation of the DPPH radical scavenging activity, total phenolic content and total flavanoid content of different solvent extracts of Catunaregam tomentosa (Blume ex DC) tirveng leaves
2021
Ahmad Syibli Othman | Syed Ahmad Tajudin Tuan Johari | Abdul Manaf Ali | Wan Amalina Wan Mamat | Muhammad Yusran Abdul Aziz
Catunaregam tomentosa(Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family, and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory, antispasmodic, antidysenteric, antifertility and immunomodulatory. In this study, the leaves were extracted using dichloromethane, ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method are 115.0±1.135, 340.8±3.209and 30.24±1.702mg GAE/g with dichloromethane, ethanol,and ethyl acetate, respectively. Total flavonoid was determined by the aluminium chloride calorimetric methodare 47.01±0.577, 56.78±1.970and 40.89±1.079mg QE/g with dichloromethane, ethanol,and ethyl acetate, respectively. Meanwhile, its antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content.The ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.07±0.51μg/mL.
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