Application of Fourier infrared spectroscopy method for quantification of foliar litter composition
2023
Skranda, Ilona | Butlers, Aldis
Foliar litter has an important role in the nutrient cycle of the forest ecosystem. Various litter fractions have different chemical composition and decomposition rate, which determines the dynamics of soil nutrient input. In addition, the proportion of litter fractions varies within the year and inter-annually depending on both biotic and abiotic factors. Therefore, knowledge of the composition of litter fractions is important for the accurate modelling of nutrient cycling. This study aimed to evaluate the application of the Fourier transform infrared spectroscopy (FTIR) method for the quantification of litter fractions to increase the efficiency of acquiring such data by avoiding the need for manual sorting of litter fractions. Mid-infrared diffuse reflectance Fourier transform spectrometer (MIR-DRIFTS) was calibrated and the elaborated quantification methods were validated by using 152 fine-milled foliar litter samples with a known composition of four fractions: pine needles; all needles and leaves; twigs; rest of litter, including seeds, flowers, and insects. Samples used were collected at three Scots pine (Pinus sylvestris L.) dominated stands over eight years. The performance of elaborated MIR-DRIFTS methods was evaluated by estimating bias from reference value and standard deviation of results obtained. The estimated combined uncertainty of litter fraction quantification results by the MIR-DRIFTS method is in a range from 12% to 15%.
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书目信息
出版者 Latvia University of Life Sciences and Technologies
ISSN 1691-5976