Exploiting Genomic Features to Improve the Prediction of Transcription Factor Binding Sites in Plants

International audience

英语. Abstract The identification of transcription factor (TF) target genes is central in biology. A popular approach is based on the location by pattern-matching of potential cis-regulatory elements (CREs). During the last few years, tools integrating next-generation sequencing data have been developed to improve the performances of pattern-matching. However, such tools have not yet been comprehensively evaluated in plants. Hence, we developed a new streamlined method aiming at predicting CREs and target genes of plant TFs in specific organs or conditions. Our approach implements a supervised machine learning strategy, which allows to learn decision rule models using TF ChIP-chip/seq experimental data. Different layers of genomic features were integrated in predictive models: the position on the gene, the DNA-sequence conservation, the chromatin state, and various cis-regulatory element footprints. Among the tested features, the chromatin features were crucial for improving the accuracy of the method. Furthermore, we evaluated the transferability of predictive models across TFs, organs and species. Finally, we validated our method by correctly inferring the target genes of key TF controlling metabolite biosynthesis at the organ-level in Arabidopsis. We developed a tool -Wimtrap- to reproduce our approach in plant species and conditions/organs for which ChIP-chip/seq data are available. Wimtrap is a user-friendly R package that supports a R-shiny web interface and is provided with pre-built models that can be used to quickly get predictions of CREs and TF gene targets in different organs or conditions in Arabidopsis thaliana, Solanum lycopersicum, Oryza sativa, and Zea mays.