Poplar under drought: Comparison of leaf and cambial proteomic responses
2011
Durand, Thomas | Sergeant, Kjell | Renaut, Jenny | Planchon, Sébastien | Hoffmann, Lucien | Carpin, Sabine | Label, Philippe | Morabito, Domenico, D. | Hausman, Jean-François | Arbres et Réponses aux Contraintes Hydriques et Environnementales (ARCHE) ; Institut National de la Recherche Agronomique (INRA) | Department Environment and Agro-Biotechnologies ; Centre de Recherche Public - Gabriel Lippmann (LUXEMBOURG) | Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF) ; Institut National de la Recherche Agronomique (INRA) | Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC) ; Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO)
International audience
显示更多 [+] 显示较少 [-]英语. The forest ecosystem is of particular importance from an economic and ecological perspective. However, the stress physiology of trees, perennial and woody plants, is far from being fully understood. For that purpose, poplar plants were exposed to drought; the plants exhibited commonly reported drought stress traits in the different plant tissues. Leafy rooted cuttings of poplar were investigated through a proteomic approach in order to compare the water constraint response of two plant tissues, namely leaf and cambium. Sampling was realized during the drought period at 2 time points with increased drought intensity and 7 days after rewatering. Our data show that there is a difference in the moment of response to the water constraint between the two tissues, cambium being affected later than leaves. In leaves, drought induced a decrease in rubisco content, and an increase in the abundance of light harvesting complex proteins as well as changes in membrane-related proteins. In the cambial tissue, the salient proteome pattern change was the decrease of multiple proteins identified as bark storage proteins. After rewatering, almost all changes in cambial proteome disappeared whereas a significant number of leaf proteins appeared to be differentially regulated only during the recovery from drought.
显示更多 [+] 显示较少 [-]