Development of 1AS.1AL-1DL durum wheat chromosome carrying Glu-D1a locus encoding high molecular weight glutenin subunits 2 + 12
2019
Coriton, Olivier | Faye, Annie | Paux, Etienne | Lemoine, Jocelyne | Huteau, Virginie | Branlard, Gerard | Jahier, Joseph | Institut de Génétique, Environnement et Protection des Plantes (IGEPP) ; Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST | Génétique Diversité et Ecophysiologie des Céréales (GDEC) ; Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]) | 123, CASDAR
International audience
显示更多 [+] 显示较少 [-]英语. High molecular weight glutenin subunits (HMW-GS) are well-known to directly influence the bread wheat quality. In a previous paper, our group reported the replacement of the locus Glu-A1 by the locus Glu-D1allele Glu-D1a encoding the HMW-GS (2-12)in a partial isohomoeoallelic lines of the French bread wheat variety cv. Courtot. We plan to exploit the novel translocated 1AS.1AL-1DL chromosome in durum wheat in order to develop durum lines presenting novel properties. The translocated chromosome was first introduced into durum wheat. However, as the distal translocated 1DL segment represents 25% (i.e., 84Mb) of the recombinant 1AL length, it appeared worthwhile to shorten it through homoeologous recombination induced by the ph1c mutation. Two rounds of recombination were made. The shortest interstitial 1DL segment detected through SNP genotyping measured 9.19Mb and could not be visualized by genomic in situ hybridization. Such reduced transfer not previously achieved hitherto provides regularity of the meiotic pairing and will allow the use of those subunits absent in durum wheat species for direct exploitability in breeding
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