Application of qPCR for plum pox virus detection during cryotherapy
Jevremović, Darko | Vasilijević, Bojana | Anđelić, Tatjana | Vujović, Tatjana
For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryo treated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR assay showed higher sensitivity in comparison to conventional RT-PCR.
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出版者 Faculty of Agronomy , Čačak (Serbia)