DNA damage in caged Gammarus fossarum amphipods: A tool for freshwater genotoxicity assessment | Dommages à l'ADN chez l'amphipode Gammarus fossarum encagés : un outil pour le suivi de la génotoxicité des milieux dulçaquicoles
2011
Lacaze, Emilie | Devaux, Alain | Mons, R. | Bony, Sylvie | Garric, Jeanne | Geffard, Adeline | Geffard, Olivier | Milieux aquatiques, écologie et pollutions (UR MALY) ; Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF) | Institut National de la Recherche Agronomique (INRA) | Université de Reims Champagne-Ardenne (URCA)
[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCA
显示更多 [+] 显示较少 [-]International audience
显示更多 [+] 显示较少 [-]英语. The aim of this study was to propose a tool for freshwater environmental genotoxicity assessment using Gammarus fossarum, a high ecologically relevant species. In a first part, gammarids were caged upstream and downstream wastewater treatment plant effluent output. The sensitivity of genotoxic responses of haemocytes, oocytes and spermatozoa was compared using the Comet assay. Spermatozoa appeared to be the most sensitive, suitable and relevant cell type for genotoxicity risk assessment. In a second part, a watershed-scale study was conducted over 2 years to evaluate the applicability of our caging procedure. The genotoxic impact of a contamination was followed, taking into account seasonal variability. DNA damage in spermatozoa exhibited low basal level and low variability in control upstream sites, providing a reliable discrimination of polluted sites. Finally, DNA damage in caged G. fossarum has been proved to be a sensitive and reproducible tool for freshwater genotoxicity assessment.
显示更多 [+] 显示较少 [-]