Detection of wildtype Merkel cell polyomavirus genomic sequence and VP1 transcription in a subset of Merkel cell carcinoma
2023
Kervarrec, Thibault | Guyétant, Serge | Berthon, Patricia | Touzé, Antoine | Samimi, Mahtab | Appenzeller, Silke | Tallet, Anne | Guillonneau, Francois | Jullie, Marie-Laure | Sohier, Pierre | Rütten, Arno | Hainaut-Wierzbicka, Ewa | Le Corre, Yannick | Blom, Astrid | Beneton, Nathalie | Bens, Guido | Nardin, Charline | Aubin, Francois | Dinulescu, Monica | Visée, Sebastien | Herfs, Michael | Houben, Roland | Schrama, David | CHU Trousseau [Tours] ; Centre Hospitalier Régional Universitaire de Tours (CHRU Tours) | Infectiologie et Santé Publique (UMR ISP) ; Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Université Paris Cité (UPCité) | Biomarqueurs et essais cliniques en Cancérologie et Onco-Hématologie (BECCOH) ; Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay | German Research Foundation | Ligue Nationale Contre le Cancer | Interdisziplinäres Zentrum für Klinische Forschung Würzburg (IZKF B-343), German Research Foundation (SCHR 1178/3-1)
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显示更多 [+] 显示较少 [-]英语. Aims: Merkel cell carcinoma (MCC) is frequently caused by the Merkel cell polyomavirus (MCPyV). Characteristic for these virus-positive (VP) MCC is MCPyV integration into the host genome and truncation of the viral oncogene Large T antigen (LT), with full-length LT expression considered as incompatible with MCC growth.Methods and results: Whole-genome sequencing of MCC/trichoblastoma again provided evidence of a trichoblastoma-derived MCC. Although an MCC-typical LT-truncating mutation was detected, we could not determine an integration site and we additionally detected a wildtype sequence encoding full-length LT. Similarly, Sanger sequencing of the combined MCC/poroma revealed coding sequences for both truncated and full-length LT. Moreover, in situ RNA hybridization demonstrated expression of a late region mRNA encoding the viral capsid protein VP1 in both combined as well as in a few cases of pure MCC.Conclusion: The data presented here suggest the presence of wildtype MCPyV genomes and VP1 transcription in a subset of MCC.
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