AP2 domain structure and protein motif features in Azerbaijan local durum and bread wheat genotypes
2023
Rustamova, Samira | Abdullayeva, Gulnar | Shahmuradov, Ilham | Huseynova, Irada
DREB proteins belonging to the superfamily of AP2/ERF plant transcription factors play an important role in the signaling network that modulates many processes, such as stress responses and plant development. In the present study, we have isolated and molecularly characterized partial DREB1 gene from Azerbaijan's local durum and bread wheat genotypes, which are distinguished by tolerance to abiotic stress factors. Analysis of amino acid sequences encoded by the putative DREB genes revealed a strongly conserved AP2/ERF domain with two conserved functional amino acids (14th valine and 19th glutamic acid) which play crucial roles in the recognition of the DNA binding site. Nuclear localization signal and conserved Ser/Thr-rich region were observed in the corresponding amino acid sequences. One α-helix and two β-sheets were detected in the secondary structure of the AP2 domain. In the protein sequence with the AP2 domain, 3 and 11 amino acid substitutions were detected in bread wheat and durum wheat, respectively. The identified sequences of the DREB1 gene from durum and bread wheat are available in the GenBank database (Accession number MZ935737.1, MZ935738.1). Characterization of the DREB genes from the stress-tolerant wheat genotypes is important for further understanding the role of this gene in the plant stress-tolerance mechanisms. Moreover, the identified single nucleotide variations associated with stress tolerance can be used in genome editing for improving crops under stress conditions.
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