Production, Purification, and Characterization of Phaseolus vulgaris Leghemoglobin a in Pichia pastoris
2022
Kim, J.Y. | Han, D.H. | Park, G.O. | Nam, S.W. | Kim, Y.H. | Kim, H.W. | Jeon, S.J.
In this study, Phaseolus vulgaris (kidney bean) leghemoglobin a (PhLba) gene was cloned into pPICZαA and expressed in Pichia pastoris to sustainably produce a heme-carrying protein for organoleptic use in plant-based meat. The recombinant PhLba protein was secreted into the culture medium in a solubilized form, and the molecular weight of the purified PhLba was estimated to be 16.5 kDa using SDS-PAGE. In addtion, the yield of recombinant PhLba holoprotein was enhanced by supplementation of the cultivation medium with hemin. This result indicates that the apo-forms of PhLba can be effectively saturated with cofactor.
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