Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery
2023
Kim, H.B. | Han, S.M. | Choi, K.C. | Lee, J.H. | Lee, S.H. | Won, Y.W. | Lim, K.S.
Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.
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