Hypocrellin B and paclitaxel-encapsulated hyaluronic acid–ceramide nanoparticles for targeted photodynamic therapy in lung cancer
2016
Chang, Ji-Eun | Cho, Hyun-Jong | Yi, Eunjue | Kim, Dae-Duk | Jheon, Sanghoon
To increase the therapeutic efficacy of photodynamic therapy (PDT) in treating lung cancer, we developed both photosensitizer and anticancer drug encapsulated hyaluronic acid–ceramide nanoparticles. Based on our previous study, a co-delivery system of photosensitizers and anticancer agents greatly improves the therapeutic effect of PDT. Furthermore, hyaluronic acid–ceramide-based nanoparticles are ideal targeting carriers for lung cancer.In vitro phototoxicity in A549 (human lung adenocarcinoma) cells and in vivo antitumor efficacy in A549 tumor-bearing mice treated with hypocrellin B (HB)-loaded nanoparticles (HB-NPs) or hypocrellin B and paclitaxel loaded nanoparticles (HB–P-NPs) were evaluated.Cell viability assay, microscopic analysis and FACS analysis were performed for the in vitro studies and HB–P-NPs showed enhanced phototoxicity compared with HB-NPs. In the animal study, the tumor volume change and the histological analysis was studied and the anticancer efficacy improved in the order of free HB<HB-NPs<HB–P-NPs.In conclusion, the combination therapy of PDT and chemotherapy, and hyaluronic acid–ceramide nanoparticle-based targeted delivery improved the effects of PDT in lung cancer in mice.
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