Epoxidation of oleic acid catalyzed by PSCI-Amano lipase optimized by experimental design
2012
Corrêa, Flávia de Abreu | Sutili, Felipe K. | Miranda, Leandro S.M. | Leite, Selma G.F. | De Souza, Rodrigo O.M.A. | Leal, Ivana C.R.
The present work focuses on the oleic acid epoxide production by using PSCI Amano Lipase as biocatalyst in the reaction. An experimental design (central composite design – CCD) adopting surface response was applied to this purpose. Reactions were performed in a shaker equipment and different variables were investigated, such as temperature (25–55°C), enzyme load (10–20wt% of oleic acid mass), hydrogen peroxide load (0.1–0.2%) and reaction time. PSCI-Amano enzyme showed its best behavior as biocatalyst after 3h of reaction at 55°C, 10% enzyme load, 0.2% hydrogen peroxide and, applying 150rpm as stirring. On these conditions, the epoxide yield was around 88%.
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