Nitrification/denitrification shaped the mercury-oxidizing microbial community for simultaneous Hg0 and NO removal
2019
Huang, Zhenshan | Wei, Zaishan | Xiao, Xiaoliang | Tang, Meiru | Li, Bolong | Zhang, Xiao
A denitrifying/nitrifying membrane biofilm reactor for simultaneous removal of Hg⁰ and NO was investigated. Hg⁰ and NO removal efficiency attained 94.5% and 86%, respectively. The mercury-oxidizing microbial community was significantly shaped by nitrification/denitrification after the supply of gaseous Hg⁰and NO continuously. Dominant genera Rhodanobacter and Nitrosomonas participated in Hg⁰ oxidation, nitrification and denitrification simultaneously. Hg⁰ oxidizing bacteria (Gallionella, Rhodanobacter, Ottowia, Nitrosomonas and etc.), nitrifying bacteria (Nitrosomonas, Rhodanobacter, Diaphorobacte and etc.) and denitrifying bacteria (Nitrosomonas, Rhodanobacter, Castellaniella and etc.) co-existed in the MBfR, as shown by metagenomic sequencing. X-ray photoelectron spectroscopy (XPS) and high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) confirmed the formation of a mercuric species (Hg²⁺) from mercury bio-oxidation. Mechanism of mercury oxidation can be described as the bacterial oxidation of Hg⁰ in which Hg⁰ serves as electron donor, NO serves as electron donor in nitrification and electron acceptor in denitrification, oxygen serves as electron acceptor.
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