An Efficient In Vitro Approach for Direct Regeneration and Callogenesis of Adhatoda vasica Nees, a Potential Source of Quinazoline Alkaloids
2017
Panigrahi, Jitendriya | Gantait, Saikat | Patel, Illa C.
The present study reports a comprehensive approach on in vitro direct regeneration and callus induction of Adhatoda vasica Nees from in vivo nodal segment (NS) explants. For direct initiation of shoots, 0.6–1.4 mg/l of N⁶-benzyladenine (BA) was employed in Murashige and Skoog (MS) medium. The earliest shoot initiation was recorded within 6 days of inoculation, whereas maximum number (7.4) and length (7.2 cm) of shoots with highest number (2.8) of leaves per shoot were recorded in MS medium plus 1.1 mg/l BA. Isolated shoots were transferred to MS medium, supplemented with diverse combinations of indole-3-butyric acid (IBA) (0.1–2.5 mg/l) and α-napthalene acetic acid (NAA) (1–3 mg/l), for the purpose of root induction. Maximum (94%) rooting was recorded in MS medium fortified with 1 mg/l IBA and 0.25 mg/l NAA (8.4 roots/shoot with 5.6 cm length). The regenerated plantlets were acclimatized initially in soil and sand (1:1; v/v) for 4 weeks recording a survival rate of > 95%. Finally, the surviving plantlets were established in sand, soil and farmyard manure (1:1:1; v/v) for another 4 weeks. For callus induction, NS explants were inoculated in MS medium fortified either with 2,4-dichlorophenoxy acetic acid (2,4-D) (0.1–2.5 mg/l) or with different combinations of BA (0.5 mg/l) and NAA (0.5–2.5 mg/l). Highest (46%) callus induction was recorded in MS medium with 1 mg/l 2,4-D that subsequently induced ~ 60 roots per callus, devoid of adventitious shoots.
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