Lipid changes due to growth-factor supplements in callus and plasma membrane-enriched fraction of rice cultures
1996
Sathishkumar, R. | Man̲ōkaran̲, Ki
Membrane-lipid composition of total callus and its plasma membrane enriched fraction of cultures of Oryza sativa grown on media supplemented with various growth factors, such as choline, putrescine and pectin, was determined. Whereas choline was growth stimulatory, putrescine and pectin inhibited growth in these cultures. Growth response due to the growth-factor supplements was associated with significant changes in the total content, as well as the relative amounts of phospholipids and galactolipids. Whereas choline supplementation led to an increase in the relative amounts of phosphatidylcholine and phosphatidylinositol, putrescine/pectin supplemented cultures showed higher level of phosphatidic acid. A plasma membrane-enriched fraction of choline-supplemented cultures had higher content of phospholipids and galactolipids, together with enriched contents of phosphatidylcholine. Results are discussed in relation to the role of membrane lipids in in vitro growth.
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