Probing the effects of hexavalent chromium exposure on histology and fatty acid metabolism in liver of Bufo gargarizans tadpoles
2020
Yang, Yijie | Wang, Wenxiang | Liu, Xiaoli | Song, Xiuling | Chai, Lihong
Hexavalent chromium is one of the major detrimental heavy metal pollutants. B. gargarizans tadpoles were treated with different concentrations of Cr⁶⁺ (0, 13, 52, 104, 208, and 416 μg Cr⁶⁺ L⁻¹) from Gs 2 to Gs 42. The effect of Cr⁶⁺ on histopathological alterations and transcript levels of fatty acid metabolism-related genes as well as fatty acids composition and content in liver were examined. Histopathological changes were observed in liver at 52, 104, 208, and 416 μg Cr⁶⁺ L⁻¹. Moreover, RT-qPCR analyses showed the downregulated mRNA levels of the genes related to fatty acid synthesis (SCD, MECR, TECR and ELOVL1) and fatty acid β-oxidation (ACOT1, PPT1, HADH and ACAA2) at 416 μg Cr⁶⁺ L⁻¹. However, the mRNA expression of fatty acid β-oxidation-related genes (ECHS1, HADHA and ACAA1) were significantly upregulated at 13, 52, 104, 208 and 416 μg Cr⁶⁺ L⁻¹. In situ hybridization revealed BSEP was expressed in hepatocyte nucleus and plasma membrane, and HSD17B12 was abundantly expressed in the plasma membrane. The HSD17B12 mRNA levels were significantly upregulated in tadpoles exposed to all Cr⁶⁺ treatment groups, while the BSEP mRNA levels were downregulated at 104, 208 and 416 μg Cr⁶⁺ L⁻¹ groups compared to control. In addition, an increase in polyunsaturated fatty acids and a decrease in monounsaturated fatty acids were found in 52, 104 and 416 μg Cr⁶⁺ L⁻¹ groups. Overall, chronic exposure to Cr⁶⁺ may suppress fatty acid synthesis, disturb fatty acid β-oxidation, aggravate disorders of hepatic function and induce hepatic impairment in B. gargarizans tadpoles.
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