Identification, phylogeny and transcript profiling of ERF family genes during temperature stress treatment in Pea (Pisum sativum L.)
2022
Sharma, Samriti | Chahal, Anuraj | Prasad, Heerendra | Walia, Abhishek | Kumar, Raj | Dobhal, Sneha
Ethylene responsive transcription factors is a superfamily of transcription factors, regulates various developmental, defense and stress responses, and their use in plant improvement require to have better understanding of these proteins. In the present study, 68 ERF genes have been selected from Pea EST raw data from various publically available databases. Phylogenetic analysis with pea ERF domain shows its close relatedness to ERF60 and ERF92. Transcriptomic analysis revealed that ERF60 and ERF92 are more specific to temperature stress tolerance. Broad phenotypic variability among the temperature stress treated and control of pea varieties, i.e. P-89, Arkel, and Azad Pea-1 were detected by using morphological descriptors. A set of seven gene-specific primers were developed based on ERF unigenes which gave 100% polymorphism. The results from present study reveal that the genes of ERF family play various roles in plants life and generated comprehensive data will be useful in conducting functional genomics. Physiological and molecular analyses of stress treated pea varieties showed that low temperature Arkel variety performed best under low temperature stress condition and could be used for crop breeding programs.
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