Diversity of pilin of serologically distinct Bacteroides nodosus

Gradin, J.L.; Stephens, J.A.; Pluhar, G.E.; Marta, K.M.; Smith, A.W.

Diversity of pilin of serologically distinct Bacteroides nodosus

1991

Gradin, J.L. | Stephens, J.A. | Pluhar, G.E. | Marta, K.M. | Smith, A.W.

Pili from 11 distinct serotypes of Bacteroides nodosus were examined for diversity of pilin polypeptide subunits among serotypes and for purity of the pilin preparations. The pilin of all 11 samples was shown to be homogeneous. Mean +/- SD molecular weight of the pilin of 7 serotypes (A198, IV, V, VI, IX, XVII, and XVIII) was 18,500 +/- 100. The pilin of serotypes I, III, and VIII had molecular weight of 17,600, 19,400, and 19,000, respectively. Serotype XV differed greatly from the other 10 serotypes in that 2 distinct polypeptide bands with molecular weight of approximately 7,800 and 6,200 were detected. We suggest that these 2 low molecular weight bands resulted from proteolytic cleavage of the pilin protein.

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