Impact of chlorfenvinphos, an organophosphate insecticide on human blood mononuclear cells (in vitro)
2012
Szatkowska, Bożena | Kwiatkowska, Marta | Michałowicz, Jaromir | SiciÅska, Paulina | Huras, Bogumiła | Bukowska, Bożena
In the present study, the investigation of the effect of chlorfenvinphos (CFVF) on necrotic and apoptotic changes as well as on selected morphological and biochemical parameters in human blood mononuclear cells were investigated. We analyzed the effect of this compound on proteins damage and free-radical formation in human blood mononuclear cells. Furthermore, changes in the size (FSC-A) and granularity (SSC-A) of human blood mononuclear cells exposed to chlorfenvinphos were assessed. In order to detect apoptosis, two testes were used including analysis of YO-PRO-1 iodide/propidium iodide and Annexin V/propidium iodide staining, which revealed that chlorfenvinphos increased the number of apoptotic cells at its highest concentration of 250μM. Chlorfenvinphos at the concentrations from 50 and 100μM increased the size and granularity of the blood mononuclear cells, respectively. Moreover, chlorfenvinphos induced the statistically significant loss of human blood mononuclear cells viability at the concentration of 250μM. Protein damage (the increase in carbonyl groups content) was provoked by CFVF at concentrations of 100μM and 250μM. Furthermore, chlorfenvinphos from relatively low concentrations of 5μM induced reactive oxygen species formation (ROS). Conclusion: The present findings provide information that chlorfenvinphos only at 250μM is harmful to human blood mononuclear cells, the concentration which may appear in the organism only as a result of acute or subacute poisoning. Lower concentration (5–50μM), which caused changes in level of ROS formation can affect human organism as a result of environmental exposure.
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