Detection of marine neurotoxins in food safety testing using a multielectrode array
2014
Nicolas, Jonathan | Hendriksen, Peter J. M. | van Kleef, Regina G. D. M. | de Groot, Aart | Bovee, Toine F. H. | Rietjens, Ivonne M. C. M. | Westerink, Remco H. S.
SCOPE: At the European level, detection of marine neurotoxins in seafood is still based on ethically debated and expensive in vivo rodent bioassays. The development of alternative methodologies for the detection of marine neurotoxins is therefore of utmost importance. We therefore investigated whether and to what extent a multielectrode array (MEA) approach can be used as an in vitro alternative for screening of marine neurotoxins potentially present in seafood. METHODS: This MEA approach utilizes rat cortical neurons comprising a wide range of ion channels/pumps and neurotransmitter receptors targeted by marine neurotoxins. We tested the effects of neurotoxic model compounds, pure marine neurotoxins, and extracts from contaminated seafood on neuronal activity of rat cortical neurons cultured on commercial 48‐well plates to increase throughput. CONCLUSION: We demonstrate that the MEA approach has a sensitivity of 88% (7/9 model compounds, 6/6 pure marine neurotoxins, and 2/2 marine neurotoxins present in seafood extracts were correctly identified) and a good reproducibility compared to existing in vitro alternatives. We therefore conclude that this MEA‐based approach could be a valuable tool for future food safety testing.
显示更多 [+] 显示较少 [-]