Characterization of nuclear microsatellite loci in the calcareous fen specialist Scorpidium cossonii (Calliergonaceae)
2011
Kophimai, Yuppayao | Cornejo, Carolina | Peintinger, Markus | Bergamini, Ariel | Scheidegger, Christoph
• Premise of the study: Nuclear microsatellite markers were developed in the fen specialist moss, Scorpidium cossonii, to study genetic diversity and genetic structure of this species in relation to land-use types, habitat fragmentation, and habitat conservation measures. • METHODS: and Results: The polymorphisms of 14 microsatellite markers were characterized. All primers were tested on 140 gametophytes collected from four populations in Switzerland. The primers amplified di- and trinucleotide repeats with three to 26 alleles per locus. The primers worked also in the two other species of the genus: 14 and 12 primers successfully amplified in S. revolvens and S. scorpioides, respectively. • CONCLUSIONS: In future studies, our primers have the potential to provide valuable information on genetic diversity, genetic structure, and on historical and recent gene flow in S. cossonii; they should also enable related research in other Scorpidium species.
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