Immunological effects of collagen and collagen peptide from blue shark cartilage on 6T-CEM cells
2017
Bu, Yongshi | Elango, Jeevithan | Zhang, Jingyi | Bao, Bin | Guo, Ruihua | Palaniyandi, Krishnamoorthy | Robinson, Jeya Shakila | Geevaretnam, Jeyasekaran | Regenstein, J. M. (Joe M.) | Wu, Wenhui
The physicochemical and in vitro mechanism of immunologic tolerance of pepsin-soluble collagen and its peptide, CII-P, from blue shark cartilage were studied. Protein patterns showed three identical (α1)3 chains, suggesting that it was a type-II collagen (CII). CII-P had high antioxidant activity and low carbohydrate content. Collagens had better biocompatibility with decreased the viability of 6T-CEM cell compared to control cells (without collagen). Immunological indices such as FAS/APO-1, cytokine, and caspase levels were higher in CII-treated 6T-CEM cells. Collagen bound to 6T-CEM cell receptors in a dose-dependent manner, and an optimum effect was observed with 10μg/mL collagen. The high carbohydrate content of CII could activate the FAS receptor, which led to increased apoptotic gene expression in 6T-CEM cells. Breakdown of 6T-CEM cell nuclei through the induction of apoptosis by CII was confirmed by fluorescence microscopy. Collagen molecular weight and glycosylation patterns were crucial factors for immunologic tolerance and 6T-CEM cellular apoptosis.
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