Development of rice bran treatment process and its use for the synthesis of polyhydroxyalkanoates from rice bran hydrolysate solution
2015
Oh, Young Hoon | Lee, Seung Hwan | Jang, Young-Ah | Choi, Jae Woo | Hong, Kyung Sik | Yu, Ju Hyun | Shin, Jihoon | Song, Bong Keun | Mastan, Shaik G. | David, Yokimiko | Baylon, Mary Grace | Lee, Sang Yup | Park, Si Jae
Rice bran treatment process for the production of 43.7kg of hydrolysate solution containing 24.41g/L of glucose and small amount of fructose from 5kg of rice bran was developed and employed to produce polyhydroxyalkanoates in recombinant Escherichia coli and Ralstonia eutropha strains. Recombinant E. coli XL1-Blue expressing R. eutropha phaCAB genes and R. eutropha NCIMB11599 could produce poly(3-hydroxybutyrate) with the polymer contents of 90.1wt% and 97.2wt%, respectively, when they were cultured in chemically defined MR medium and chemically defined nitrogen free MR medium containing 10mL/L of rice bran hydrolysate solution, respectively. Also, recombinant E. coli XL1-Blue and recombinant R. eutropha 437-540, both of which express the Pseudomonas sp. phaC1437 gene and the Clostridium propionicum pct540 gene could produce poly(3-hydroxybutyrate-co-lactate) from rice bran hydrolysate solution. These results suggest that rice bran may be a good renewable resource for the production of biomass-based polymers by recombinant microorganisms.
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