The influence of commonly used selective agents on the growth of Listeria monocytogenes
1999
Jacobsen, C.N.
The effects, upon addition of various selective agents to a rich growth medium (BHIEM) on the growth of Listeria monocytogenes and non-Listeria were studied in microtitre wells. A combination of nalidixic acid (0.1 g/l), acriflavine (0.02 g/l) and fosfomycin (0.1 g/l) added to the medium (BHIEM(NAF)) was found to be the least inhibitory to Listeria while at the same time, effectively inhibitory to non-Listeria organisms. Recovery of heat injured Listeria monocytogenes in BHIEM, BHIEM(NAF) and the two traditional Listeria-media, UVM I and II, was compared. Recovery in BHIEM was obtained within 24 h of incubation at 30 degrees C, whereas recovery in BHIEM(NAF) took up to 48 h. Growth and recovery in UVM I and II never reached the same level as in BHIEM or BHIEM(NAF).
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