Extracellular Carbohydrate Esterase from the Basidiomycete Coprinopsis cinerea Released Ferulic and Acetic Acids from Xylan
2010
HASHIMOTO, Kohsuke | Kaneko, Satoshi | Yoshida, Makoto
cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, α-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-β-1,4-xylanase.
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书目信息
Bioscience, biotechnology, and biochemistry
卷
74
期
8
页码
1722
- 1724
ISSN
1347-6947
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
其它主题
Coprinopsis cinerea; Basidiomycete; Carbohydrate esterase; Komagataella pastoris
语言
英语
注释
Nal-light
类型
Text; Journal Article
2024-02-27
MODS