Regulatory Role of Nitric Oxide in Neutrophil Apoptosis
2008
Stepovaya, Ye. A | Zhavoronok, T. V | Starikov, Yu. V | Bychkov, V. A | Chasovskih, N. Yu | Starikova, Ye. G | Petina, G. V | Novitsky, V. V | Ryazantseva, N. V
Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H₂O₂, selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H₂O₂ led to activation of the apoptotic program in neutrophils, which was seen from increased content of Bax protein in the cells and increased number of apoptotic cells in the culture. Increased content of annexin-positive cells after incubation of neutrophil culture with NO synthase inhibitor suggests involvement of NO in the regulation of neutrophil apoptosis under conditions of oxidative stress, while L-arginine prevented H₂O₂-induced programmed cell death.
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